I agree with schiele, GLaDOS was actually funny in a "dark" sort of way. This computer is just a douche with a funny accent that insults you all the time. I muted it after the /second/ time I died, only because you did a pretty good job on making the voice sound synthesized.
I know this was made in 72 hours, but there are sooooo many things wrong with this game. Before I figured out there was more than one room, I turned off the life support and killed everyone, since I thought it was the only choice. You should clue players in to this, instead of just saying something vague like "push that red button", of which there are none... At least make a "restart" button, for godsakes.
Also, you should provide a little information on this "protomolecule". It sounds nothing like a germ, but that's what the she-asshole computer tells you /after/ you die. Would have been really nice if there were information on the proto-molecule somewhere, so I wouldn't have to restart the game *yet again* after freeing it...
If the oxygen is "green" (plentiful), then how come waking people up kills you? Wouldn't it just go to "yellow"?
At least you made a walkthrough... You probably wouldn't need one, though, if the game itself gave the player a little bit more information (perhaps about the consequences of their actions, too... Although I admit "release carnivores" was pretty obviously going to kill you, so no need for more information on that one LOL), and things made a bit more sense. I definitely see potential in this game, since, well who doesn't love a "stranded spaceship" scenario?